2-way anova glm model  (SAS institute)

Journal: International Journal of Molecular Sciences

Article Title: Accelerated Wound Closure of Deep Partial Thickness Burns with Acellular Fish Skin Graft

doi: 10.3390/ijms22041590

Figure Lengend Snippet: Skin Barrier Function Measurements. ( A ) TransEpidermal Water Loss (TEWL) measures the barrier properties of the epidermis. At each time point, three TEWL measurements were taken for each of the twenty four wounds and then averaged. (# = p < 0.0001; @ = p < 0.05 for normal skin vs. treatment groups). ( B ) Hydration levels of wounds on follow-up days represented in Log scale (a log transformation was performed on hydration data because measurements ranged from 10 to 10,000). The hydration measures the water content of the wounds. Five measurements were obtained for each wound at each time point and averaged. (# = p < 0.001 and @ = p < 0.05 for normal skin vs. CTPs; * = p < 0.05 for FBD vs. FSG). For A and B, significance was detected using a 2-way ANOVA mixed effects model and a Tukey post-hoc test. Fetal bovine dermis (FBD); Fish skin graft (FSG).

Article Snippet: For TEWL and hydration, a 2 way ANOVA mixed effects model with Tukey post-hoc test was performed in GraphPad due to missing data points.

Techniques: Transformation Assay

Journal: International Journal of Molecular Sciences

Article Title: Accelerated Wound Closure of Deep Partial Thickness Burns with Acellular Fish Skin Graft

doi: 10.3390/ijms22041590

Figure Lengend Snippet: Laser Speckle Imaging. ( A ) Two representative wounds are shown for both treatment groups. Digital images at day 14 and the corresponding laser speckle images (LSI) throughout the experiment are shown. The heatmap scale is shown below in which blue represents low perfusion and red if highly perfused. Note that the perfusion in uninjured skin is a sky blue and as the wounds heal by day 60, the borders of the wound are no longer obvious. ( B ) Quantitation of LSI measurements represented as a fold change above the normal perfusion around each wound and normalized to the growth controls on each animal at the designated time point (# = p < 0.005 for normal skin vs. treatment groups; * = p < 0.05 for FSG vs. FBD as determined by 2-way repeated measures ANOVA with Tukey post-hoc test, n = 12). Fetal bovine dermis (FBD); Fish skin graft (FSG).

Article Snippet: For TEWL and hydration, a 2 way ANOVA mixed effects model with Tukey post-hoc test was performed in GraphPad due to missing data points.

Techniques: Imaging, Quantitation Assay